Imaging a Windows 10 OS to a new disk can potentially cause issues with the Microsoft key or license, depending on the type of media used to create the image. If the operating system image was created using retail or evaluation media, you will likely need to specify a product key during the installation process, as the installation may stop if a key is required and not provided.
However, if the image was created using Volume License media or certain OEM media, you might not need to specify a product key during the deployment, as these types of media can allow for bare metal deployment without requiring a key. It's essential to know the type of installation media used to create the operating system image to avoid any licensing issues during installation.
To ensure a smooth installation, it's recommended to test the deployment first by deploying only one node with that operating system image to confirm whether a product key is required or not.